BOBS-PWEE50 Style Sample Holder

A 3D printed sample holder for thin film spectroscopy to measure blood hemoglobin affinity in the PWEE50 style for the BOBS™ machine by Loligo Systems.

A small quantity (on the order of microliters) of blood is placed between two layers of Glad Go-Between Freezer Film, this is then stretched over the sample window via the rubber o-ring and tamp (cylindrical object). The rod is used to position the o-ring over the film and window, and then the tamp stretches the o-ring across the film to create the thinnest possible blood sample, allowing for rapid oxygenation and deoxygenation.

Example of film stretched over sample window.
Slicer preview for 3D printing on the Bambu Lab X1C.

Download and edit the 3D models here via Onshape.

Massive thank you to Peter Frappell the creator of the PWEE50.

This project was in collaboration with duckDNA lab.